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연구성과 > 금주의 우수논문

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SCI Article

Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human
성명 신흥수()
소속 공과대학 생명공학과
캠퍼스
우수선정주 2018년 12월 2째주
Author Kim, Se-jeong (Dept Bioengn); Park, Jaesung (Dept Bioengn); Byun, Hayeon (Dept Bioengn); Park, Young-Woo (Dept Bioengn); 이동윤 (Dept Bioengn); 신흥수 (Dept Bioengn); Kim, Se-jeong (Plus Future Biopharmaceut Human Resources Trainin); Park, Jaesung (Plus Future
Corresponding Author Info Shin, H (reprint author), Hanyang Univ, Dept Bioengn, 222 Wangsimri Ro, Seoul 04763, South Korea.
E-mail 이메일 아이콘hshin@hanyang.ac.kr
Document Type Article
Source BIOMATERIALS Volume:188 Issue: Pages:198-212 Published:2019
Times Cited 0
External Information http://dx.doi.org/10.1016/j.biomaterials.2018.10.025
Abstract Stem cell spheroids have been studied extensively in organoid culture and therapeutic transplantation. Herein, hydrogels with an embossed surface (HES) were developed as an all-in-one platform that can enable the rapid formation and culture of a large quantity of size-controllable stem cell spheroids. The embossed structure on the hydrogel was adjustable according to the grit designation of the sandpaper. Human adipose-derived stem cells (hADSCs) were rapidly assembled into spheroids on the hydrogel, with their size distribution precisely controlled from 95 +/- 6 pm to 181 +/- 15 mu m depending on surface roughness. The hADSC spheroids prepared from the HES demonstrated expression of sternness markers and differentiation capacity. In addition, HES-based spheroids showed significantly greater VEGF secretion than spheroids grown on a commercially available low-attachment culture plate. Exploiting those advantages, the HES-based spheroids were used for 3D bioprinting, and the spheroids within the 3D-printed construct showed improved retention and VEGF secretion compared to the same 3D structure containing single cell suspension. Collectively, HES would offer a useful platform for mass fabrication and culture of stem cell spheroids with controlled sizes for a variety of biomedical applications.
Web of Science Categories Engineering, Biomedical; Materials Science, Biomaterials
Funding National Research Foundation of Korea; Korean government (MEST) [NRF-2016R1A2B3009936]
Language English
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