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SCI Article

Fluorescing aptamer-gold nanosensors for enhanced sensitivity to bisphenol A
성명 김영필()
소속 자연과학대학 생명과학과
캠퍼스
우수선정주 2018년 05월 1째주
Author Lee, Eun-Song (Dept Life Sci); Kim, Gae Baik (Dept Life Sci); Ryu, Su-Hyun (Dept Life Sci); Lee, Jin-Won (Dept Life Sci); Gye, Myung Chan (Dept Life Sci); Kim, Young-Pil (Dept Life Sci); Kim, Hyeon (Inst Pharmaceut Sci & Technol); Yoo, Hye Hyun (Inst Pha
Corresponding Author Info Gye, MC; Kim, YP (reprint author), Hanyang Univ, Dept Life Sci, Seoul 04763, South Korea.
E-mail 이메일 아이콘ypilkim@hanyang.ac.kr
Document Type Article
Source SENSORS AND ACTUATORS B-CHEMICAL Volume:260 Issue: Pages:371-379 Published:2018
Times Cited 0
External Information http://dx.doi.org/10.1016/j.snb.2018.01.018
Abstract An insufficient sensitivity of aptamer-integrated colorimetric gold nanoparticles (AuNPs) is a common challenge in the detection of environmental chemicals. We report the use of a modified aptamer/AuNP-based sensor in conjunction with a fluorescing single-stranded DNA aptamer for the sensitive detection of bisphenol A (BPA), which is a major endocrine disruptor (EDC). While an anti-BPA single-stranded DNA aptamer was bound with BPA, a weak fluorescence signal was observed upon the addition of SYBR Green-I (SG-I), which is specific to a duplex region of the free aptamer. This reaction was accompanied by a sub-sequent aggregation of unmodified AuNPs (citrate-stabilized AuNPs) at high salt concentrations, leading to a color change from reddish to purple. In contrast, the absence of BPA elicited a high fluorescence signal from SG-I and produced no color change in the AuNP solution even upon the addition of salt ions. Unlike aptamers that are labeled at their ends with fluorophores, our strategy gave rise to neither a loss of target-binding affinity nor a perturbation of the AuNP colorimetry. Thus, a quantitative analysis witha broad dynamic range was achieved by combining the fluorescent and colorimetric measurements at BPA concentrations ranging over four orders of magnitude. Our approach also yielded a greater detection sensitivity (as low as 9 pg.mL(1)) than classical AuNP colorimetry or other aptamer-combined methods Moreover, this method enabled the detection of BPA in a small fraction extracted from thermal paper with a high specificity among EDCs. We anticipate that this approach will facilitate further advances in the design of traditional AuNP colorimetric sensors using different aptamers for chemical sensing in the environmental and clinical fields. (c) 2018 Elsevier B.V. All rights reserved.
Web of Science Categories Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
Funding National Research Foundation of Korea(NRF) - Ministry of Science, ICT & Future Planning [2015M3C8A6A06012737]; NRF - Ministry of Education [2012R1A6A1029029]
Language English
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