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SCI Article

Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR
성명 배상수()
소속 자연과학대학 화학과
캠퍼스
우수선정주 2018년 04월 4째주
Author Park, Jeongbin (Dept Chem); 배상수 (Dept Chem); Bae, Sangsu (Res Inst Convergence Basic Sci);
Corresponding Author Info Bae, S (reprint author), Hanyang Univ, Dept Chem, Seoul 04763, South Korea.; Bae, S (reprint author), Hanyang Univ, Res Inst Convergence Basic Sci, Seoul 04763, South Korea.
E-mail 이메일 아이콘sangsubae@hanyang.ac.kr
Document Type Article
Source BIOINFORMATICS Volume:34 Issue:6 Pages:1077-1079 Published:2018
Times Cited 0
External Information http://dx.doi.org/10.1093/bioinformatics/btx695
Abstract Following the type II CRISPR-Cas9 system, type V CRISPR-Cpf1 endonucleases have been found to be applicable for genome editing in various organisms in vivo. However, there are as yet no web-based tools capable of optimally selecting guide RNAs (gRNAs) among all possible genome-wide target sites. Here, we present Cpf1-Database, a genome-wide gRNA library design tool for LbCpf1 and AsCpf1, which have DNA recognition sequences of 5'-TTTN-3' at the 5' ends of target sites. Cpf1-Database provides a sophisticated but simple way to design gRNAs for AsCpf1 nucleases on the genome scale. One can easily access the data using a straightforward web interface, and using the powerful collections feature one can easily design gRNAs for thousands of genes in short time.
Web of Science Categories Biochemical Research Methods; Biotechnology & Applied Microbiology; Computer Science, Interdisciplinary Applications; Mathematical & Computational Biology; Statistics & Probability
Funding Next-Generation BioGreen 21 Program [PJ01119201]; Korea Healthcare technology RD Project [HI16C1012]; Basic Science Research Program [NRF-2016R1D1A1B0 3934811]
Language English
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