메인메뉴바로가기

한양대학교 산학협력단

홈으로 바로가기 > 연구정보 > 연구성과 > Recent Articles

연구성과 > Recent Articles

  • 한양대학교 기관으로 출판된 최신 SCI급 논문현황 (Web Of Science에서 제공되는 자료)
  • 매주 1회 업데이트
페이스북 로그인트위터 로그인카카오톡 로그인메일 전송
view

SCI Article

PRMT8 Controls the Pluripotency and Mesodermal Fate of Human Embryonic Stem Cells By Enhancing the P
Author Kim, Rae-Kwon (Dept Life Sci);
Corresponding Author Info Cha, HJ (reprint author), Sogang Univ, Dept Life Sci, 35 Baekbeom Ro, Seoul 04107, South Korea.; Moon, SH (reprint author), Konkuk Univ, Sch Med, Dept Med, 120 Neungdong Ro, Seoul 05029, South Korea.
E-mail 씠硫붿씪 븘씠肄moon@kku.ac.kr 씠硫붿씪 븘씠肄 hjcha@sogang.ac.kr
Document Type Article
Source STEM CELLS Volume:35 Issue:9 Pages:2037-2049 Published:2017
Times Cited 0
External Information PDF 븘씠肄http://dx.doi.org/10.1002/stem.2642
Abstract Basic fibroblast growth factor (bFGF) supplementation is critical to maintain the pluripotency of human pluripotent stem cells (hPSCs) through activation of PI3K/AKT, rather than MEK/ERK pathway. Thus, elaborate molecular mechanisms that preserve PI3K/AKT signaling upon bFGF stimulation may exist in hPSCs. Protein arginine methyltransferase 8 (PRMT8) was expressed and then its level gradually decreased during spontaneous differentiation of human embryonic stem cells (hESCs). PRMT8 loss- or gain-of-function studies demonstrated that PRMT8 contributed to longer maintenance of hESC pluripotency, even under bFGF-deprived conditions. Direct interaction of membrane-localized PRMT8 with p85, a regulatory subunit of PI3K, was associated with accumulation of phosphoinositol 3-phosphate and consequently high AKT activity. Furthermore, the SOX2 induction, which was controlled by the PRMT8/PI3K/AKT axis, was linked to mesodermal lineage differentiation. Thus, we propose that PRMT8 in hESCs plays an important role not only in maintaining pluripotency but also in controlling mesodermal differentiation through bFGF signaling toward the PI3K/AKT/SOX2 axis.
Web of Science Categories Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology
Funding Korea Health Technology R&D Project through Korea Health Industry Development Institute (KHIDI); Ministry of Health Welfare [HI15C3051, HI14C3365]; National Research Foundation of Korea (NRF) [2017R1A2A2A05000766]
Language English
페이스북 로그인트위터 로그인카카오톡 로그인메일 전송 리스트