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  • 한양대학교 기관으로 출판된 최신 SCI급 논문현황 (Web Of Science에서 제공되는 자료)
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SCI-E Article

Effects of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas)
Author Kim, Su-Jin (Dept Biomed Sci); Kim, Chang-Hoon (Dept Biomed Sci); Kim, Kye-Seong (Dept Biomed Sci); Kim, Kye-Seong (Coll Med);
Corresponding Author Info Kim, KS (reprint author), Hanyang Univ, Grad Sch Biomed Sci & Engn, Dept Biomed Sci, 222 Wangsimni Ro, Seoul 04763, South Korea.; Kim, KS (reprint author), Hanyang Univ, Coll Med, Seoul, South Korea.; Hong, SH (reprint author), Kangwon Natl Univ, Sch Med,
E-mail shhong@kangwon.ac.kr ks66kim@hanyang.ac.kr
Document Type Article
Source TISSUE ENGINEERING AND REGENERATIVE MEDICINE Volume:14 Issue:2 Pages:179-185 Published:2017
Times Cited 0
External Information http://dx.doi.org/10.1007/s13770-017-0031-8
Abstract Pluripotent stem cells (PSCs) are a useful source of cells for exploring the role of genes related with early developmental processes and specific diseases due to their ability to differentiate into all somatic cell types. Recently, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system has proven to be a robust tool for targeted genetic modification. Here, we generated miR-451-deficient PSCs using the CRISPR/Cas9 system with PCR-based homologous recombination donor and investigated the impact of its deletion on self-renewal and hematopoietic development. CRISPR/Cas9-mediated miR-451 knockout did not alter the gene expressions of pluripotency, cellular morphology, and cell cycle, but led to impaired erythrocyte development. These findings propose that a combination of PSCs and CRISPR/Cas9 system could be useful to promote biomedical applications of PSCs by elucidating the function and manipulating of specific miRNAs during lineage specification and commitment.
Web of Science Categories Cell & Tissue Engineering; Engineering, Biomedical
Funding Medical Research Center [2008-0062287]; Basic Research Lab Program - NRF of the Ministry of Science, ICT & Future Planning, Republic of Korea [2015R1A4A1038666]
Language English
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